《植物生理学报》 2017, 53(11): 1963-1970

不同甘蔗在梢腐病菌侵染下氮代谢相关指标的变化

王泽平, 李毅杰, 梁强, 李长宁, 韦金菊, 刘璐, 林善海^*

广西农业科学院甘蔗研究所/中国农业科学院甘蔗研究中心/农业部广西甘蔗生物技术与遗传改良重点实验室, 南宁530007

通信作者：林善海；E-mail: 745784546@qq.com

摘　要：

本文以甘蔗‘粤糖94/128’ (YT 94/128)和‘桂糖37号’ (GT 37)作为研究材料, 采用针刺接种法, 分析了不同甘蔗在梢腐病菌侵染下氮代谢相关指标的变化。结果表明, 未接种状态下, YT 94/128叶片的核糖核酸(RNA)、可溶性蛋白(SP)、硝态氮(NO₃^- -N)、铵态氮(NH₄⁺ -N)含量和叶片硝酸还原酶(NR)、谷氨酰氨合成酶(GS)、谷氨酸合成酶(GOGAT)活性都要高于GT37, 且都表现出稳中有升的变化规律。受到梢腐病菌侵染后, GT 37叶片RNA含量显著下降, GS、GOGAT活性以及SP含量下降速度显著快于YT 94/128。数据显示两类材料中氮代谢各类指标存在不同程度相关性, 也存在氨基酸组分差异。由此可见, 梢腐病菌侵染可直接导致甘蔗叶片RNA降解, NR、GS以及GOGAT活性下降, SP下降, 游离氨基酸(AA)总量上升。氨基酸不同组分与甘蔗建立抗梢腐病菌侵染机制密切相关。

关键词：甘蔗; 梢腐病; Fusarium verticillioides; 氮代谢; 互作

收稿：2017-03-28   修定：2017-11-01

资助：广西自然科学基金(2016GXNSFBA380046、2015jjBA-30013)、广西农业科学院科技发展基金(桂农科2017YM01)和广西重点研发计划项目(桂科AB16380126、桂科AB16380157)。 * 通讯作者(E-mail: 745784546@qq.com)。

Change of nitrogen metabolic indexs in different sugarcane varieties inoculated with pokkah boeng disease pathogen

WANG Ze-Ping, LI Yi-Jie, LIANG Qiang, LI Chang-Ning, WEI Jin-Ju, LIU Lu, LIN Shan-Hai^*

Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences/Sugarcane Research Center, Chinese Academy of Agricultural Science/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Nanning 530007, China

Corresponding author: LIN Shan-Hai; E-mail: 745784546@qq.com

Abstract:

This experiment used two sugarcane (Saccharum officinarum) varieties YT 94/128 and GT 37 as tested materials with syringe inoculation to study the physiological changes of nitrogen metabolism under pokkah boeng disease (PBD). The results showed that under non inoculated condition, the contents of RNA, soluble protein (SP), NO₃^- -N、NH₄⁺ -N and the activities of nitrate reductase (NR), glutamine synthetase (GS) and glutamate synthase (GOGAT) in YT 94/128 were much higher than those in GT 37, and all showed a steady rise. After infection the contents of RNA in GT 37 leaves decreased significantly, and the decrease rates of GS, GOGAT activities and SP content were faster than those in YT 94/128. And the data showed there were different degrees of correlation among various indicators of nitrogen metabolism, and there were also differences in amino acid components in the two sugarcane varieties. The infection of PBD pathogen lead to degradation of RNA, the decrease of NR, GS, GOGAT activities and SP content, and the increase of free amino acid in sugarcane leaves. Different components of amino acids were closely related to the establishment of resistance mechanism of sugarcane against PBD pathogen.

Key words: sugarcane; pokkah boeng disease; Fusarium verticillioide; nitrogen metabolism; interaction